dc.contributor.author |
Durkin, Shannon M. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:16:43Z |
|
dc.date.accessioned |
2019-09-08T02:29:09Z |
|
dc.date.available |
2011-01-31T14:16:43Z |
|
dc.date.available |
2019-09-08T02:29:09Z |
|
dc.date.created |
2000 |
en_US |
dc.date.issued |
2000 |
en_US |
dc.identifier |
45416129 |
en_US |
dc.identifier.other |
b1865292x |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1865292 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6105 |
|
dc.description |
1 v. (various pagings) : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2000. |
en_US |
dc.description |
Includes bibliographical references (leaves 69-72). |
en_US |
dc.description.abstract |
The study of catabolite repression in Neurospora crassa examines
regulation of genetic expression in the presence of various carbon sources.
Certain mutant strains of N. crassa do not display preferential expression of
genes for metabolizing preferred carbon sources. An example is the sorbose
resistant sor-4 strain.
A cotransformation experiment was devised to determine if the gene
relieving the sor-4 mutant strain of catabolite repression was complementary
to ere-I, a gene thought to be involved in the events leading to catabolite
repression. Neurospora crassa conidia were converted to spheroplasts in
order to render them competent to take up DNA. Conidia from wild type
(74A) and mutant (sor-4) strains were transformed with both the cre-l gene
and a gene encoding resistance to Hygromycin B, an antibiotic used as a
selectable marker. The transformed colonies were further examined for
morphology, and it was determined that the sor-4 colonies were not
converted back to wild type phenotype. Although resistance to the antibiotic
was conferred on both wild type and mutant cells, a Southern blot showed
that copies of cre-l were not integrated into the genome of sor-4
Neurospora crassa. Cotransformation did not give evidence of
complementation of the cre-l gene and the sor-4 mutation. |
en_US |
dc.description.statementofresponsibility |
by Shannon M. Durkin. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0697 |
en_US |
dc.subject.classification |
Master's Theses no. 0697 |
en_US |
dc.subject.lcsh |
Neurospora crassa. |
en_US |
dc.title |
Complementation of the sor-4 gene of Neurospora Crassa / |
en_US |
dc.type |
Thesis |
en_US |