dc.contributor.author |
Marshall, Eva M. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:16:46Z |
|
dc.date.accessioned |
2019-09-08T02:31:07Z |
|
dc.date.available |
2011-01-31T14:16:46Z |
|
dc.date.available |
2019-09-08T02:31:07Z |
|
dc.date.created |
1999 |
en_US |
dc.date.issued |
1999 |
en_US |
dc.identifier.other |
b1840571x |
en_US |
dc.identifier.uri |
http://www.ohiolink.edu/etd/view.cgi?ysu999018116 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1840571 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6109 |
|
dc.description |
viii,84 leaves ; ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 1999. |
en_US |
dc.description |
Includes bibliographical references (leaves ). |
en_US |
dc.description.abstract |
The biosynthesis of uridine-5' -monophosphate (UMP) can occur through
two pathways. The first and most common is the de novo pathway, while the
second and more unique pathway is the thymidine salvage pathway. The fungus
Neurospora crassa, unlike many organisms, contains both but utilizes the second
of these pathways to convert thymidine to uracil.
My research focuses on the fourth enzyme of the thymidine salvage
pathway, iso-orotate decarboxylase. Preliminary characterization of IDCase was
determined by a radioactivity based assay using 14 C to label the substrate lOA.
This decarboxylation assay measures the amount of IDCase activity by detecting
the amount of radio-labeled CO2 released from lOA in the reaction at a specified
time point.
Wild type N. crassa strains had the highest specific enzyme activity of 2.5
nmol/min/mg when grown in a shake culture for 18 hours at 30°C. We have tested
iso-orotate decarboxylase levels at various time points in the N. crassa life cycle.
We have also examined the rate of induction of IDCase activity in the
presence of nitrate as well as in the N. crassa strains containing the 2TI:1 and nit-2
mutations. As uracil has been shown to be an inducer of the IDCase levels and
may therefore act as an inducer for the thymidine salvage pathway. |
en_US |
dc.description.statementofresponsibility |
by Eva M. Marshall. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0648 |
en_US |
dc.subject.classification |
Master's Theses no. 0648 |
en_US |
dc.subject.lcsh |
Theses (Master's) |
en_US |
dc.title |
Measurement of Iso-orotate Decarboxylase activity in Neurospora Crassa, / |
en_US |
dc.type |
Thesis |
en_US |