dc.contributor.author |
Schmidt, Brian P. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:17:00Z |
|
dc.date.accessioned |
2019-09-08T02:31:13Z |
|
dc.date.available |
2011-01-31T14:17:00Z |
|
dc.date.available |
2019-09-08T02:31:13Z |
|
dc.date.created |
1999 |
en_US |
dc.date.issued |
1999 |
en_US |
dc.identifier.other |
b18502027 |
en_US |
dc.identifier.uri |
http://www.ohiolink.edu/etd/view.cgi?ysu999616997 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1850202 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6126 |
|
dc.description |
x, 88 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 1999. |
en_US |
dc.description |
Includes bibliographical references (leaves ). |
en_US |
dc.description.abstract |
Natural Killer (NK) cells are white blood cells that participate in the direct
cellular elimination of tumor and virus-infected cells. NK cell function is determined
through the use of cytotoxicity assays. Our study investigates the use of an LDH assay
(Promega Cytotox 96® LDH assay) to characterize murine NK cells. Four target cell
lines were tested for their effectiveness in this assay. Studies using 51Cr release assays
have shown that MPC II and YAC-l cell lines are targeted for lysis by murine NK cells
(positive controls) and that L1210 and P815 cell lines are not targeted for NK cell lysis
(negative controls). The optimal number of target cells (total LDH two-fold higher than
background) was 5,000 cells/lOOl-ll for P815 and YAC-l, 10,000 cells/lOOl-ll for L1210
and MPC II. The 4 and 24 spontaneous release values (percentage of the total LDH
release) were as follows: P815: 0 and 46.651 %, YAC-l: 028.985%, L121O: 0 and
12.661 %, and MPC II: 13.490% and 41.542%. Experimental cytotoxicity assays were
also performed, with the optimal number of target cells incubated with NK cells at
various effector (NK) to target cell ratios. P815 experienced a low % cytotoxicity
(0.369%) and was an effective negative control cell line. MPC II exhibited a high %
cytotoxicity (25.8%) and was an effective positive control cell line. No specific LDH
release was seen with YAC-l target cells at the effector to target cell ratios used in these
studies. The LDH cytotoxicity assay is useful in determining murine NK cell
cytotoxicty. The spontaneous and total release data along with the results of the LDH
cytotxicity assays obtained from these positive and negative control target cell lines will
aid in the determination of the functional activity of an NK cell preparation. |
en_US |
dc.description.statementofresponsibility |
by Brian P. Schmidt. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0669 |
en_US |
dc.subject.classification |
Master's Theses no. 0669 |
en_US |
dc.subject.lcsh |
Theses (Master's) |
en_US |
dc.title |
The use of lactate dehydrogenase for the detection of murine natural killer cell function, / |
en_US |
dc.type |
Thesis |
en_US |