dc.contributor.author |
Damico, Nicole. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:17:31Z |
|
dc.date.accessioned |
2019-09-08T02:29:15Z |
|
dc.date.available |
2011-01-31T14:17:31Z |
|
dc.date.available |
2019-09-08T02:29:15Z |
|
dc.date.created |
2000 |
en_US |
dc.date.issued |
2000 |
en_US |
dc.identifier |
47215844 |
en_US |
dc.identifier.other |
b18792686 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1879268 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6153 |
|
dc.description |
viii, 98 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2000. |
en_US |
dc.description |
Includes bibliographical references (leaves 94-98). |
en_US |
dc.description.abstract |
NK cells, a component of our innate immune system, are responsible
for the elimination of virus-infected and tumor cells which bypass detection
by T cells. In these studies, we attempted to create a NK cell hybridoma
clone, which would provide a testable pool of NK cells. Mouse splenocytes
were fused with P3X mouse non-secreting myeloma cells in the presence of
polyethylene glycol (PEG). Following HAT selection, the cells were tested
for the presence of the mouse NK cell surface antigen DX5. After labeling
with biotinylated anti-mouse DX5 and Strepavidin-FITC in the presence of
rat anti-mouse CD16/CD32 (Fe Block), the mixed lymphocyte cell pool
which yielded the highest amount of mean fluorescence (257.17), was cell
pool C6. C6 was then subcloned, and the clones were retested for the
presence ofDX5 and absence ofCD3. The data analysis, however, was
hindered by the presence of autofluorescence in the hybridoma cell line. In
an attempt to correct the autofluorescence of the hybridomas, two
permeabilzing detergents, n-Octyl-B-D-Glucopyr anoside (OG), and
Cytoperm/Ctyofix™ were used with various concentrations (.01 f.!g/ml to
10f.!g/ml) of the quenching dye trypan blue. Because of the low binding
affinity of the anti-DX5 antibody and the inability to compensate for cellular
autofluorescence a pure NK cell hybridoma clone could not be identified. |
en_US |
dc.description.statementofresponsibility |
by Nicole Damico. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0693 |
en_US |
dc.subject.classification |
Master's Theses no. 0693 |
en_US |
dc.subject.lcsh |
Hybridomas. |
en_US |
dc.subject.lcsh |
Clone cells. |
en_US |
dc.title |
Preparing and cloning a natural killer cell hybridoma / |
en_US |
dc.type |
Thesis |
en_US |