dc.contributor.author |
White, Jason. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:18:43Z |
|
dc.date.accessioned |
2019-09-08T02:30:26Z |
|
dc.date.available |
2011-01-31T14:18:43Z |
|
dc.date.available |
2019-09-08T02:30:26Z |
|
dc.date.created |
2002 |
en_US |
dc.date.issued |
2002 |
en_US |
dc.identifier |
51198307 |
en_US |
dc.identifier.other |
b19012470 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1901247 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6233 |
|
dc.description |
viii, 83 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2002. |
en_US |
dc.description |
Includes bibliographical references (leaves 78-83). |
en_US |
dc.description.abstract |
The quinic acid cluster of Neurospora crassa provides an interesting model
for studying genetics. Its unique operon-like organization provides a good
opportunity to study the eukaryotic mechanism of activation and repression of the
cluster responsible for utilizing quinic acid as a carbon source. The activator protein,
qa-1F, elevates levels of transcription by binding at a conserved sequence that occurs
at least 14 times within the cluster. The activator, qa-1F, has one of its highest
affinities for the activator-binding site that regulates its own transcription. This site
occurs at -144 in relationship to the start site of transcription of qa-lF . By isolating
this sequence using peR, and cloning into a RF M13mp18 baculovirus vector, the
vector will be utilized to produce single-stranded infectious phage particles with our
cloned binding site. Once these infectious phage particles are isolated, site-directed
mutagenesis will be performed using the kunkel method to change specific
conserved bases. These mutants can be transformed back into Neurospora where the
significance of these changes will be better understood. |
en_US |
dc.description.statementofresponsibility |
by Jason White. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0758 |
en_US |
dc.subject.classification |
Master's Theses no. 0758 |
en_US |
dc.subject.lcsh |
Neurospora crassa. |
en_US |
dc.title |
Site-directed mutagenesis of the -144 activator-binding site of qa-1F in neurospora crassa / |
en_US |
dc.type |
Thesis |
en_US |