A recombinant GST-EMBP440 
fusion protein from sea urchin embryos that has myosin binding capabilities

Vrable, David Joseph.

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dc.contributor.author	Vrable, David Joseph.	en_US
dc.date.accessioned	2011-01-31T14:19:02Z
dc.date.accessioned	2019-09-08T02:28:36Z
dc.date.available	2011-01-31T14:19:02Z
dc.date.available	2019-09-08T02:28:36Z
dc.date.created	1997	en_US
dc.date.issued	1997	en_US
dc.identifier	273050935	en_US
dc.identifier.other	b17819593	en_US
dc.identifier.uri	http://jupiter.ysu.edu/record=b1781959	en_US
dc.identifier.uri	http://hdl.handle.net/1989/6259
dc.description	vi 66 leaves : ill. ; 29 cm.	en_US
dc.description	Includes bibliographical references (leaf 41).	en_US
dc.description	Thesis (M.S.)--Youngstown State University, 1997.	en_US
dc.description.abstract	A 53 kDal. (53K) native protein was isolated from sea urchin egg extract. The properties of this protein suggests that it may be a titin-like protein. Previous studies have shown that this protein has myosin light chain kinase activity. The antibody to native 53 kDal. was used to select recombinant GST-EMBP440 protein from a cDNA library. GST-EMBP440 protein was used in this study. This study confirms the myosin binding activity of GST-EMBP440 protein. Two binding assays were utilized to demonstrate myosin binding by GST-EMBP440 protein. A blot binding assay utilizing immunodetection suggested a lack of 53 kDal. binding. Results from a glutathione-agarose affinity precipitation assay indicated binding of the GST-EMBP440 fusion protein to myosin. The findings therefore suggest GST-EMBP440 protein may indeed be a myosin binding protein.	en_US
dc.description.sponsorship	Youngstown State University. Dept. of Biology.	en_US
dc.description.statementofresponsibility	by David Joseph Vrable.	en_US
dc.language.iso	en_US	en_US
dc.relation.ispartofseries	Master's Theses no. 0583	en_US
dc.subject.classification	Master's Theses no. 0583	en_US
dc.subject.lcsh	Sea urchins--Embryos.	en_US
dc.subject.lcsh	Recombinant proteins.	en_US
dc.title	A recombinant GST-EMBP440 fusion protein from sea urchin embryos that has myosin binding capabilities	en_US
dc.type	Thesis	en_US

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A recombinant GST-EMBP440 fusion protein from sea urchin embryos that has myosin binding capabilities