dc.contributor.author |
Nestich, Scott J. |
en_US |
dc.date.accessioned |
2011-01-31T14:19:27Z |
|
dc.date.accessioned |
2019-09-08T02:29:10Z |
|
dc.date.available |
2011-01-31T14:19:27Z |
|
dc.date.available |
2019-09-08T02:29:10Z |
|
dc.date.created |
1997 |
en_US |
dc.date.issued |
1997 |
en_US |
dc.identifier |
273051234 |
en_US |
dc.identifier.other |
b17756455 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1775645 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6278 |
|
dc.description |
ix, 88 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 1997 |
en_US |
dc.description |
Includes bibliographical references (leaves 82-88). |
en_US |
dc.description.abstract |
Mitogen-activated protein (MAP) kinases (also called extracellularsignal-
regulated kinases [ERKs]) are activated in response to a wide array of
extracellular stimuli in many different cell types (Gardner et aI., 1993).
ERKs are serine/threonine kinases that are important signal transducers
during the Go to G1 transition point where the cell in a resting state of the cell cycle enters the first growth stage before DNA synthesis and also during the G2 to M phase where the cell continues to grow to the point where the nucleus and cytoplasm divide (Ruderman, 1993). ERKs are important
regulators of such cellular processes as cell growth, cell division, and cell differentiation. My study compares ERKI from a human recombinant
GST/ERKI fusion protein and ERKI from sea urchin (Lytechinus variegatus
or Strongylocentrotus purpuratus) egg extracts with the use of mouse
antibodies produced against the recombinant protein. The major finding in
this study was that epitope cross-reactivity occurred in sea urchin extracts in the molecular weight range expected for the recombinant GST/ERKI fusion protein on a Western blot. |
en_US |
dc.description.sponsorship |
Youngstown State University. Dept. of Biology. |
en_US |
dc.description.statementofresponsibility |
by Scott J. Nestich. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0575 |
en_US |
dc.subject.classification |
Master's Theses no. 0575 |
en_US |
dc.subject.lcsh |
Immunoglobulin genes. |
en_US |
dc.subject.lcsh |
Genetic recombination. |
en_US |
dc.title |
Antibody production to human
recombinant ERK1: comparison of human ERK1 and sea urchin ERK1 |
en_US |
dc.type |
Thesis |
en_US |