dc.contributor.author |
Landfried, Daniel A. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Chemistry. |
en_US |
dc.date.accessioned |
2011-01-31T14:19:47Z |
|
dc.date.accessioned |
2019-09-08T02:32:42Z |
|
dc.date.available |
2011-01-31T14:19:47Z |
|
dc.date.available |
2019-09-08T02:32:42Z |
|
dc.date.created |
2003 |
en_US |
dc.date.issued |
2003 |
en_US |
dc.identifier.other |
b19352888 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1935288 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6291 |
|
dc.description |
xi, 62 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2003. |
en_US |
dc.description |
Includes bibliographical references (leaves 37-38). |
en_US |
dc.description.abstract |
Iso-orotate decarboxylase (lDCase) is the final enzyme in the thymine salvage
pathway, which is possessed by few organisms. A possible mechanism for this
enzymatic decarboxylation is similar to a ~-keto acid decarboxylation, but multiple
mechanism schemes are possible for the non-enzymatic decarboxylation reaction.
Protein taken from Rhodotorula glutinis cells was assayed for IDCase by decarboxylation
of the labeled [carboxy_14C] iso-orotate to 14C02. Attachment of a known inhibitor to an
agarose column allowed for a five-fold purification ofIDCase, with a seven percent
recovery. A eDNA library of R. glutinis was constructed and converted to a plasmid
library to screen for the gene responsible for the IDCase protein. Isolation of the gene
responsible for IDCase could help increase effectiveness of IDCase isolation. However,
colonies of E. coli grown on restrictive media, designed to select for the presence of
IDCase, did not contain active IDCase, according to assay data. For the non-enzymatic
decarboxylation, spontaneous decarboxylation of iso-orotic acid does not occur at a
measurable rate, but the BU4Am+-iso-orotate salt is decarboxylated at a rate of2.45xlO-6
sec-I. This rate is much slower than the non-published data of iso-orotic acid in water. |
en_US |
dc.description.statementofresponsibility |
by Danial A. Landfried. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0797 |
en_US |
dc.subject.classification |
Master's Theses no. 0797 |
en_US |
dc.subject.lcsh |
Decarboxylation. |
en_US |
dc.title |
Iso-orotate decarboxylase : progress toward protein isolation, gene screening, and the non-enzymatic decarboxylation mechanism / |
en_US |
dc.type |
Thesis |
en_US |