dc.contributor.author |
D'Elia, Thomas J. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:20:07Z |
|
dc.date.accessioned |
2019-09-08T02:33:11Z |
|
dc.date.available |
2011-01-31T14:20:07Z |
|
dc.date.available |
2019-09-08T02:33:11Z |
|
dc.date.created |
2004 |
en_US |
dc.date.issued |
2004 |
en_US |
dc.identifier.other |
b19594409 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1959440 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6311 |
|
dc.description |
ix, 75 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2004. |
en_US |
dc.description |
Includes bibliographical references (leaves 73-75). |
en_US |
dc.description.abstract |
119 Escherichia coli isolates from nine different animal sources were subjected to
denaturing gradient gel electrophoresis (DGGE) to determine sequence variations within
the 16S-23S intergenic spacer region (ISR) of the rrnB ribosomal operon. The ISR was
analyzed to determine if E. coli isolates from various animal sources could be
differentiated from each other. In E. coli, the 16S-23S ISR has been demonstrated to
consist of non-essential sequences that are subject to frequent insertion or deletion events
that may allow for differences between different isolates. DNA isolated from the E. coli
animal sources was PCR amplified to isolate the rrnB operons. To prevent PCR
amplification of all seven E. coli ribosomal operons by PCR amplification by using
universal primers, sequence specific primers were utilized for the rrnB operon. An
additional primer set was then used on these amplimers to prepare samples of the 16S23S
ISR for DGGE. DGGE results show the presence of 40 unique ISR sequences from
all of the samples. The highest rate of unique banding patterns, 60%, was observed for
humans. The genetic profiles established by the PCR-DGGE method revealed a high
genetic diversity for the E. coli isolates tested. There was also very little correlation
between the ISR profiles created by the DGGE bands between the host sources. These
findings suggest that the 16S-23S ISR may contain some host specificity, and that the
high diversity of the E. coli isolates may allow for the assessment of environmental
samples to distinguish similarities. |
en_US |
dc.description.statementofresponsibility |
by Tom D'Elia. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0823 |
en_US |
dc.subject.classification |
Master's Theses no. 0823 |
en_US |
dc.subject.lcsh |
Escherichia coli. |
en_US |
dc.subject.lcsh |
Bacterial genetics. |
en_US |
dc.title |
Source tracking of Escherichia coli using the 16S-23S intergenic spacer region of the rrnB ribosomal operon / |
en_US |
dc.type |
Thesis |
en_US |