dc.contributor.author |
DelFraino, Brian J. |
en_US |
dc.date.accessioned |
2011-01-31T14:20:09Z |
|
dc.date.accessioned |
2019-09-08T02:35:14Z |
|
dc.date.available |
2011-01-31T14:20:09Z |
|
dc.date.available |
2019-09-08T02:35:14Z |
|
dc.date.created |
2003 |
en_US |
dc.date.issued |
2003 |
en_US |
dc.identifier.other |
b19695275 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1969527 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6314 |
|
dc.description |
viii, 44 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2003. |
en_US |
dc.description |
Includes bibliographical references (leaves 43-44). |
en_US |
dc.description.abstract |
Orotidylate decarboxylase (ODCase) is the final enzyme in the de novo
biosynthetic pathway of pyrimidine biosynthesis. The chemical mechanism by which
ODCase catalyzes the formation of uridylate (UMP) to orotidylate (OMP) is not yet
apparent since it follows no common decarboxylation routes. Our group has suggested a
model for this decarboxylation that involves proton donation to the transition state in a
concerted ylide/zwitterions mechanism. Yeast ODCase was purified of any
contaminating proteins. Complete synthesis and characterization of transition-analogues
was performed which act and were used as inhibitors to explore the enzyme active site.
IH NMR of spectroscopy of ODCase in complex with 6-thiocarboxamidoruidine-5'monophosphate
reveals a new downfield signal, possible identifying hydrogen bonds
between the enzyme and the inhibitor. Our proposed catalytic mechanism and the results
obtained from the experiment are to be discussed in this thesis. |
en_US |
dc.description.statementofresponsibility |
by Brian J. DelFraino. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0804 |
en_US |
dc.subject.classification |
Master's Theses no. 0804 |
en_US |
dc.subject.lcsh |
Decarboxylases. |
en_US |
dc.subject.lcsh |
Decarboxylation. |
en_US |
dc.subject.lcsh |
Chemistry, Organic. |
en_US |
dc.subject.lcsh |
Pyrimidines. |
en_US |
dc.title |
OMP decarboxylase : the reaction mechanism and the binding of an isosteric inhibitor / |
en_US |
dc.type |
Thesis |
en_US |