dc.contributor.author |
Brown, Donald E. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:20:24Z |
|
dc.date.accessioned |
2019-09-08T02:35:12Z |
|
dc.date.available |
2011-01-31T14:20:24Z |
|
dc.date.available |
2019-09-08T02:35:12Z |
|
dc.date.created |
2004 |
en_US |
dc.date.issued |
2004 |
en_US |
dc.identifier.other |
b1969510x |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1969510 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6323 |
|
dc.description |
vii, 81 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2004. |
en_US |
dc.description |
Includes bibliographical references (leaves 80-81). |
en_US |
dc.description.abstract |
Wangiella dermatitidis is a pathogenic fungus capable of expressing three distinct
modes of cellular development. These forms include yeast, multicellular, and mould
phases. In particular, the latter two morphologies are induced from the yeast phase by
specific environmental conditions. Temperature-sensitive mutants, designated Mc
strains, have also been derived that grow as yeasts at 25°C, but develop in the
multicellular phenotype when incubated at 37°C. The latter strongly resembles that
tissue form of certain pathogenic fungi phylogenetically related to W dermatitidis. These
observations form the basis ofthe following hypothesis: the conversion from the yeastlike
budding phase to the multicellular growth pattern is associated with the expression of
specific proteins. Therefore, the present study seeks to establish a proteome map ofW
dermatitidis for subsequent studies targeting virulence-associated proteins.
Yeast-phase cultures ofW dermatitidis were incubated for 24 hours at 25°C and
37°C prior to homogenization. Protein components of the homogenate were resolved by
two-dimensional gel electrophoresis, then analyzed by fluorescence imaging using Sypro
Ruby Stain. A proteomic profile was created for the wild-type strain 8656 at both
temperatures as well as for the mutant strain Mc3. Distinct protein spots separated in the
profiles were excised and are being further identified by mass spectrometry. The
collective results ofthe present study demonstrate the potential of standard proteomic
techniques for dissecting the molecular mechanisms of virulence in W dermatitidis. |
en_US |
dc.description.statementofresponsibility |
by Donald E. Brown. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0835 |
en_US |
dc.subject.classification |
Master's Theses no. 0835 |
en_US |
dc.subject.lcsh |
Pathogenic fungi. |
en_US |
dc.subject.lcsh |
Proteomics. |
en_US |
dc.subject.lcsh |
Fungi. |
en_US |
dc.title |
Proteomic profile of Wangiella dermatitidis / |
en_US |
dc.type |
Thesis |
en_US |