dc.contributor.author |
Masullo, Pam. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:20:27Z |
|
dc.date.accessioned |
2019-09-08T02:35:10Z |
|
dc.date.available |
2011-01-31T14:20:27Z |
|
dc.date.available |
2019-09-08T02:35:10Z |
|
dc.date.created |
2004 |
en_US |
dc.date.issued |
2004 |
en_US |
dc.identifier.other |
b19692675 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1969267 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6329 |
|
dc.description |
ix, 90 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2004. |
en_US |
dc.description |
Includes bibliographical references (leaves 80-90). |
en_US |
dc.description.abstract |
Staphylococcus aureus continues to pose a major health threat, most prevalent in
the immunocompromised, despite the development ofantibiotics. Due to the increased
antibiotic resistance seen with this pathogen another means oftreatment is warranted.
Ninety-eight percent of the clinical isolates ofS aureus are encapsulated, eighty percent
of which are capsular polysaccharide (CP) type 5 or 8. CPs are thought to shield S
aureus from agglutination by antibodies to cell wall structures. For this reason,
encapsulated strains are poorly phagocytosed by polymorphonuclear leukocytes.
However, antibodies specific for the CP have been shown to be efficient in inducing
phagocytosis of encapsulated strains. S aureus remains a problem for patients with
decreased immunologic function because these individuals are often unable to form
immune responses to carbohydrate molecules. Preformed antibodies against the CP ofS
aureus would aid these patients in eliminating the bacterium. Presented in this study is
the first step to the production oftherapeutic antibodies, the isolation and purification of
S aureus CP. The CP was removed from the bacterium by autoclaving the bacterial cell
suspension. The CP was then purified by enzymatic digestion and anion exchange
chromatography. In this study, several methods are tested for their ability to detect the CP
in the anion exchange column eluate. The CP, a reducing sugar, was ultimately detected
by its ability to reduce Red Tetrazolium to an intensely colored diformazan whose
production was measured spectrophotometrically. The CP, which was determined to be
free ofteichoic acid contamination, will be used in further studies to produce preformed
antibodies against S aureus CPs. |
en_US |
dc.description.statementofresponsibility |
by Pam Massullo. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0837 |
en_US |
dc.subject.classification |
Master's Theses no. 0837 |
en_US |
dc.subject.lcsh |
Staphylococcus aureus infections--Prevention. |
en_US |
dc.subject.lcsh |
Staphylococcus aureus. |
en_US |
dc.subject.lcsh |
Polysaccharides. |
en_US |
dc.title |
Purification of Staphylococcus aureus capsular polysaccharide serotype 5 / |
en_US |
dc.type |
Thesis |
en_US |