dc.contributor.author |
Raidel, Scott Michael. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:20:49Z |
|
dc.date.accessioned |
2019-09-08T02:33:24Z |
|
dc.date.available |
2011-01-31T14:20:49Z |
|
dc.date.available |
2019-09-08T02:33:24Z |
|
dc.date.created |
1997 |
en_US |
dc.date.issued |
1997 |
en_US |
dc.identifier.other |
b17881651 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1788165 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6354 |
|
dc.description |
xii60 leaves ill. ; |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 1997. |
en_US |
dc.description |
Includes bibliographical references (leaves ). |
en_US |
dc.description.abstract |
Many microrganisms, III the presence of a preferred carbon
source, repress genes which are used to metabolize other carbon
sources, a process call carbon catabolite repression. Carbon catabolite
repreSSIOn has been shown to operate within the quinic acid utilization
pathway (qa) of Neurospora crassa. The mechanisms acting to cause
this repression remain unknown. However, a strain of N. crassa
containing a deletion of the qa -1 S repressor gene showed that some q a
genes remained slightly repressed while others remained highly
repressed, in the presence of a preferred carbon source. One of these
directly repressed genes is the gene coding for the qa-1 F activator
protein.
To investigate this phenomenon the qa-1 S-qa-1 F intergenic regIOn
of Neurospora africana was chosen for study. N. africana was chosen
because the entire qa gene sequence of N. crassa is known and provided
a comparison between the two species. First, subclones of plasmid pRl,
which contained a 3.8 kb insert known to contain the qa-1S-qa-1F
intergenic region of N. africana, were constructed. Southern blot
analysis of the subclones plasmid pRXl and pRX2 revealed that the
entire qa-1 S-qa-1 F intergenic region was contained within plasmid
pRX2. Further sequence analysis revealed the existence of portions of
the qa-1 F and qa-1 S genes and the location the intergenic region within
the original 3.8 kb insert. |
en_US |
dc.description.statementofresponsibility |
by Scott Michael Raidel. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0589 |
en_US |
dc.subject.classification |
Master's Theses no. 0589 |
en_US |
dc.subject.lcsh |
Theses (Master's) |
en_US |
dc.title |
The localization of the qa-lS-qa-IF Intergenic Region of Neurospora africana, / |
en_US |
dc.type |
Thesis |
en_US |