A biochemical characterization of the plasma membrane of the myxamoeba and swarm cells of the myxomycete Didymium iridis

Vitullo, John C

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dc.contributor.author	Vitullo, John C
dc.contributor.other	Youngstown State University, degree granting institution.
dc.contributor.other	Youngstown State University. Department of Biology.
dc.date.accessioned	2021-03-22T18:46:13Z
dc.date.available	2021-03-22T18:46:13Z
dc.date.issued	1978
dc.identifier.other	b13694492
dc.identifier.other	945976991
dc.identifier.uri	https://jupiter.ysu.edu:443/record=b1369449
dc.identifier.uri	http://hdl.handle.net/1989/16047
dc.description	viii, 50 leaves : illustrations ; 28 cm Thesis M.S. Youngstown State University 1978. Includes bibliographical references (leaves 46-50).	en_US
dc.description.abstract	The plasma membrane of the myxamoeba and warm cells of the Myxomycete Didymium iridis was biochemically characterized. Analysis of membrane proteins by SDS-polyacrylamide gel electrophoresis stained with Coomassie Blue revealed twelve components ranging in molecular weight from 24,000-200,000. The two slowest migrating components were also stained by the periodic acid - Schiffs (PAS) procedure indicating the presence of glycoproteins (GP) in these bands. The lipid component was separated into major classes by column chromatography and further fractionated by thin-layer chromatography. Fivephospholipids were resolved. Although two remain unidentified, it appears that phosphatidyl choline (PC), phosphatidyl ethanolamine (PE), and phosphatidyl insitol (PI) are present while the lone glycolipid resolved is a derivative of monogalactosyldiacylglycerol. The membrane neutral lipid is present as sterol esters as shown by gas-liquid chromatography and infrared spectroscopy. The sterols most likely to be present include cholestranol, stigmasterol, and ergasterol.	en_US
dc.description.sponsorship	Youngstown State University. Department of Biology.	en_US
dc.language.iso	en_US	en_US
dc.publisher	[Youngstown, Ohio] : Youngstown State University, 1978.	en_US
dc.relation.ispartofseries	Master's Theses;no. 0209
dc.subject	Didymium (Fungi)	en_US
dc.subject	Myxomycetes.	en_US
dc.subject	Biochemistry.	en_US
dc.title	A biochemical characterization of the plasma membrane of the myxamoeba and swarm cells of the myxomycete Didymium iridis	en_US
dc.type	Thesis	en_US

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