Determination of the major urinary 17-ketosteroids including pregnanediol and 17-ketogenic steroids by gas-liquid chromatography

Abstract

A gas-liquid chromatography (GLC) procedure is described for estimating major urinary 17-ketosteroids including pregnanediol and 17-ketogenic steroids without formation of their silylated derivatives. The separative and quantitative capabilities of GLC in determining certain urinary steroids are superior to the colorimetric methods in that; frequently, clinically significant individual alterations in steroids metabolites are masked by normal values obtained by analysis for total steroid groups. By making use of the resolving and measuring capabilities of GLC, several urinary steroid metabolites may be simultaneously assayed. The methods undertaken in the study can be applied in multiple samples, enabling the procedures to be used in routine clinical assay. Each analytical step has been made optimal, resulting in simple and rapid methods for determining the steroid metabolites in urine.