Abstract:
A characterization of the Hep-2 epidermoid carcinoma cell line was conducted to determine the functional differences between Hep=2 cells and a suspected contaminant, HeLa cervical carcinoma. Parameters utilized to measure differences between the two cell lines were based on chromosome number, serum and pH requirements, rate of growth, and isoenzyme analysis.
Results were consistent with earlier reports indicating that Hep-2 and HeLa cells that identically migrating bands on cellulose acetate for phosphoglucose isomerase (PGI) and glucose-6-phosphate dehydrogenase (G6PD). Significant differences were noted between the cell lines in regard to growth requirements, generation time, and chromosome number.