Quantitative cytophotometric analysis of nuclear DNA concentrations by use of cresyl violet acetate versus basic fuchsin

Bidinotto, Andrew C.

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dc.contributor.author	Bidinotto, Andrew C.
dc.contributor.other	Youngstown State University, degree granting institution.
dc.contributor.other	Youngstown State University. Department of Biology.
dc.date.accessioned	2021-04-26T15:33:27Z
dc.date.available	2021-04-26T15:33:27Z
dc.date.issued	1993
dc.identifier.other	B2267567x
dc.identifier.other	1197993134
dc.identifier.uri	https://jupiter.ysu.edu:443/record=b2267567
dc.identifier.uri	http://hdl.handle.net/1989/16202
dc.description	vi, 88 leaves : illustrations ; 29 cm M.S. Youngstown State University 1993. Includes bibliographical references (leaves 84-88).	en_US
dc.description.abstract	Cresyl violet, a basic, oxazine dye, was used in conjunction with the Feulgen reaction to study its staining properties and possible stoichiometric properties in comparison to basic fuchsin, which is a stoichiometric stain. The two-wavelength method of microspectrophotometry was employed to measure nuclear DNA concentrations in liver tissue, sperm, plasmodium, and myxamoeba cells. It was determined that cresyl violet is specific and stoichiometric for nuclear DNA and complexes with hydrolytic formed aldehyde groups on the DNA molecule. Furthermore, it was also demonstrated that the use of cresyl violet acetate can readily be used to study subtle nuclear DNA changes which occur during the cell cycle.	en_US
dc.description.sponsorship	Youngstown State University. Department of Biology.	en_US
dc.language.iso	en_US	en_US
dc.publisher	[Youngstown, Ohio] : Youngstown State University, 1993.	en_US
dc.relation.ispartofseries	Master's Theses;no. 0483
dc.subject	Cytophotometry.	en_US
dc.subject	DNA -- Analysis.	en_US
dc.title	Quantitative cytophotometric analysis of nuclear DNA concentrations by use of cresyl violet acetate versus basic fuchsin	en_US
dc.type	Thesis	en_US

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