dc.contributor.author |
Hunter, Lindsay A. |
|
dc.contributor.other |
Youngstown State University. Department of Biology. |
|
dc.date.accessioned |
2021-06-30T16:51:15Z |
|
dc.date.available |
2021-06-30T16:51:15Z |
|
dc.date.issued |
2006 |
|
dc.identifier.other |
B19926212 |
|
dc.identifier.other |
123501727 |
|
dc.identifier.uri |
https://jupiter.ysu.edu:443/record=b1992621 |
|
dc.identifier.uri |
http://hdl.handle.net/1989/16371 |
|
dc.description |
vi, 70 leaves : ill ; 29 cm.
Thesis (M.S.)--Youngstown State University, 2006.
Includes bibliographical references (leaves 68-70). |
en_US |
dc.description.abstract |
Mitochondrial genomes vary greatly in structure and size from organism to organism. The mechanisms for replicating mitochondrial DNA (mtDNA) are not yet completely understood. Saccharomyces cerevisiae, baker's yeast, is a model organism utilized to study mtDNA replication. An issue arises using it for research since two forms are commonly used: the wild-type form, called rho+, and a form that has undergone massive mtDNA deletions, called rho-. Since rho- cells only contain a fragment of the wild-type mitochondrial genome amplified to an equivalent mass, it is of concern that these strains may replicate in an unusual manner, akin to an SOS response. Using neutral/neutral two-dimensional (2-D) gel electrophoresis, this study presents evidence to support a mtDNA replication mechanism that is common to both rho+ and rho- strains. Both intact and restriction enzyme-digested mitochondrial genomes were examined.
The most widely-accept model of mtDNA replication in S. cerevisiae is the strand-asynchronous asymmetric model. This mechanism would generate primarily single-stranded replication intermediates. 2-D gel analysis of rho+ and rho- strains revealed both single-stranded and double-stranded replication intermediates along with potential RNA-dependent species. This suggests that more models of replication are occurring in addition to the strand-asynchronous one. These potential models include a rolling circle mechanism and also one that is dependent on recombination. Recombination-dependent replicating has been previously reported in mammals, but this is the first evidence presented for it in yeast. |
en_US |
dc.description.sponsorship |
Youngstown State University. Department of Biology. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses;no. 0937 |
|
dc.subject |
Mitochondrial DNA. |
en_US |
dc.subject |
DNA replication. |
en_US |
dc.subject |
Saccharomyces cerevisiae. |
en_US |
dc.title |
Comparison of replication structures in mutant and wild-type mitochondrial DNA of the yeast Saccharomyces cerevisiae |
en_US |
dc.type |
Thesis |
en_US |