Investigation of site-specific metal-catalyzed oxidation of proteins using dipeptides as model compounds /

Kwolek, Kathleen A.; Youngstown State University. Dept. of Chemistry.

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dc.contributor.author	Kwolek, Kathleen A.	en_US
dc.contributor.author	Youngstown State University. Dept. of Chemistry.	en_US
dc.date.accessioned	2011-01-31T14:16:25Z
dc.date.accessioned	2019-09-08T02:55:19Z
dc.date.available	2011-01-31T14:16:25Z
dc.date.available	2019-09-08T02:55:19Z
dc.date.created	1998	en_US
dc.date.issued	1998	en_US
dc.identifier.other	b18135456	en_US
dc.identifier.uri	http://www.ohiolink.edu/etd/view.cgi?ysu997720041	en_US
dc.identifier.uri	http://jupiter.ysu.edu/record=b1813545	en_US
dc.identifier.uri	http://hdl.handle.net/1989/6074
dc.description	xiii, 90 leaves : ill. ; 28 cm.	en_US
dc.description	Thesis (M.S.)--Youngstown State University, 1998.	en_US
dc.description	Includes bibliographical references (leaves ).	en_US
dc.description.abstract	Although oxidative damage to proteins has been a subject of intense investigation, very little has been elucidated regarding the site-specificity of this damage due to reactive oxygen species. The extremely reactive hydroxyl radical should cause damage to the polypeptide in the vicinity of its reaction with metal ions bound to the chain. This paper investigates the site-specificity of metal-catalyzed oxidation of dipeptides using metal ions and hydrogen peroxide to generate the hydroxyl radical. A variety of conditions (different dipeptides, metal ions, temperatures and pH) were explored. Ultraviolet spectroscopy and thin layer chromatography were used to monitor reactions. Purification of reaction products was attempted through lyophilization. Endeavors to separate the mixture after reaction used percolation column chromatography and high performance liquid chromatography.	en_US
dc.description.statementofresponsibility	by Kathleen A. Kwolek.	en_US
dc.language.iso	en_US	en_US
dc.relation.ispartofseries	Master's Theses no. 0622	en_US
dc.subject.classification	Master's Theses no. 0622	en_US
dc.subject.lcsh	Theses (Master's)	en_US
dc.title	Investigation of site-specific metal-catalyzed oxidation of proteins using dipeptides as model compounds /	en_US
dc.type	Thesis	en_US

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