dc.contributor.author |
Mosher, Jennifer J. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:20:01Z |
|
dc.date.accessioned |
2019-09-08T02:32:53Z |
|
dc.date.available |
2011-01-31T14:20:01Z |
|
dc.date.available |
2019-09-08T02:32:53Z |
|
dc.date.created |
2002 |
en_US |
dc.date.issued |
2002 |
en_US |
dc.identifier.other |
b1944672x |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1944672 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6305 |
|
dc.description |
vii, 82 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2002. |
en_US |
dc.description |
Includes bibliographical references (leaves 72-78). |
en_US |
dc.description.abstract |
Abstract-Microbial Activity
To examine potential for bioremediation of industrially contaminated sediments in the
Mahoning River of northeast Ohio, microbial activity measurements were made by
estimating dehydrogenase activity using INT. Dehydrogenases take part in respiration
and is fundamental to microbial activity. As !NT accepts electrons, it is reduced to a red
colored formazan (INTF), which allows for rate ofreduction determination by
colorimetric analysis. Attempts using previously published methods for this technique
were unsuccessful due to background chemical reactions from high levels ofPAHs and
metals in the sediments. Major modifications were necessary to circumvent these
reactions. To find a solvent that did not chemically reduce INT, the following solvents
were examined: acetonitrile, methanol, ethanol, N,N-dimethylformamide,
dichloromethane, formaldehyde and ethyl acetate. Acetonitrile had the highest extraction
efficiency and least chemical interference. Temperature trials were performed at 4,22,
40,60 and 95°C. The INT reduction was inhibited at 95°C indicative of biological
activity. Various INT concentrations were also tested, with a 1.08 mM solution having
the least variance and did not require dilution for analysis. Two and sixty minute
incubations were performed. Two-minute incubations showed initial activity and rate,
while sixty minutes gave overall activity. The method reported here yields a simple,
quick, inexpensive, and precise estimation ofmicrobial activity.
Abstract- River Sediment Survey
The Mahoning River, located in northeastern Ohio, has received direct industrial
effluents for over eighty years that have accumulated in the sediments. This study
compares the distribution of microbial activities, microbial biomass, toxicity and levels of
polycyclic aromatic hydrocarbons (PAHs) in subsurface sediments. Microbial activities
were measured via dehydrogenase reduction of iodonitrotetrazolium chloride to a
formazan dye. Microbial biomass was determined by lipid phosphate extraction.
Toxicity assays were performed using a modified basic solid phase test with
MicrotoxOmni from Azur Environmental Inc. The PAHs were extracted using USEPA
organic extraction method #3550 and quantified in a gas chromatograph/mass
spectrometer as per USEPA method #8270. The results show microbial activities and
microbial biomass higher in anthropogenically-contaminated sediments, which is
indicative of potential for use of indigenous microbes for bioremediation. |
en_US |
dc.description.statementofresponsibility |
by Jennifer J. Mosher. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0742 |
en_US |
dc.subject.classification |
Master's Theses no. 0742 |
en_US |
dc.subject.lcsh |
River sediments--Mahoning River Valley (Ohio and Pa.)--River sediments--Toxicology--Mahoning River Valley (Ohio and Pa.)--Organic water pollutants--Toxicology--Mahoning River Valley (Ohio and Pa.) |
en_US |
dc.title |
A bioloigcal and chemical comparison of impacted subsurface sediments of the lower Mahoning River / |
en_US |
dc.type |
Thesis |
en_US |