Expression of Penicillium marneffei glucan synthase genes in response to cell-wall stressors

Digital.Maag

Expression of Penicillium marneffei glucan synthase genes in response to cell-wall stressors

Show full item record

Title: Expression of Penicillium marneffei glucan synthase genes in response to cell-wall stressors
Author: Eisnaugle, Sarah
Abstract: Penicillium marneffei is an opportunistic, dimorphic fungal pathogen that causes penicilliosis in AIDS patients. In an effort to genetically characterize this organism, Kummasook et al. developed an Agrobacterium tumefaciens-mediated transformation system in 2010. This system allowed for the production of thousands of P. marneffei mutant strains that could be further assessed in order to assess gene-to-function relationships. In 2014, Suwunnakorn et al., characterized one of these mutants, I231, and determined the presence of an interrupted yakA gene which codes for a dual-specificity tyrosine phosphorylation-regulated protein kinase. They determined that the yakA mutant produced fewer conidia, possessed increased chitin content, and exhibited unique cell-wall stress responses when compared to wild-type P. marneffei. The present study seeks to investigate these stress responses in the yakA mutant, a F4 wild-type strain, and the yakA complement strain CY21 at a genetic level. Using qRT-PCR, expression of three glucan synthase genes (fksP, ags2, and kre6) was quantified using the benA beta-tubulin gene as a reference gene for normalization. The three strains were subjected to cell-wall stressors including the anionic dye Congo red, the detergent sodium dodecyl sulfate, and the glucan synthase-specific antifungal drug caspofungun and assessed for glucan synthase gene expression. The resulting gene expressions indicated that the yakA mutant actually possessed relatively larger stress responses than the wild-type, indicating that the yakA gene and its resulting protein may be responsible for the regulation of glucan synthase gene expression in response to cell-wall stress. This reasoning is reinforced by the presence of increased chitin in the yakA mutant, a characteristic common to fungal mutants lacking proper glucan regulation in their cell-walls.
Description: xii, 83 leaves : illustrations ; 29 cm
URI: http://hdl.handle.net/1989/11704
Date: 2015

Files in this item

File Name Size Format View
b21968251.pdf 1.361Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record