Effect of probiotics on human gastrointestinal microbial communities

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Effect of probiotics on human gastrointestinal microbial communities

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Title: Effect of probiotics on human gastrointestinal microbial communities
Author: Lisko, Daniel
Abstract: The human microbiome is home to roughly 100 trillion microbial, which have been shown to assist in various host physiological functions. Research shows that probiotic consumption may benefit host health. Therefore this is a two part study: project I is a study determining the effects of yogurt (probiotic) consumption on GI microbial structure and diversity, and project II is a study examining the effects of probiotic pill consumption has on stress response (cortisol) for healthy human test subjects exposed to a stressor. In chapter II we used terminal restriction fragment length polymorphism, Sanger sequencing, and qPCR to characterize microbial communities of the GI tract. Non-metric multidimensional scaling analyses, Shannon Wiener diversity index values, and qPCR (targeting Lactobacillus) analysis showed a temporal influence on microbial community, diversity and absolute abundance of Lactobacillus on human test subjects that consumed 250g of yogurt per day for 42 consecutive days. In chapter III, we quantitated Lactobacillus using qPCR and determined stress response (via cortisol) using a salivary enzyme immune assay (EIA). Results from this study showed a decrease in stress response and an increase in the relative abundance of Lactobacillus in individuals that consumed one probiotic pill per day for 30 consecutive days. In comparison, the control group showed no changes in stress response or Lactobacillus. Results from this study suggest the potential for probiotics as therapeutic treatment for host physiological function and stress response. However, further research is necessary to determine the effects probiotics have on human test subject.
Description: ix, 90 leaves : illustrations ; 29 cm
URI: http://hdl.handle.net/1989/11712
Date: 2015

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