Protein profiling in Wangiella dermatitidis

Abstract

Wangielle dermatitdis, a dematiaceous fungus, exhibits three distinct morphologies: monliform or true hyphae, unicellular budding yeasts, or multicellular forms. This study was set up to establish a proteomic protein profile of the wild type form of W. dermatitidis. It is hypothesized that there will be significant differences in protein expression between the yeast grown at the permissive temperature of 25°C and restrictive temperature of 37°C. Yeast cultures of W. dermatitidis were cycled and incubaded for 48 hours at 25°C and 37­°C before harvesting. The yeast cells were isolated using Tri Reagent and sonication. The proteins were then separated using two-dimensional gel electrophoresis and Coomassie Blue staining. Proteins similarities and differences were analyzed using PDQuest imaging software. The significant differences that were found could lead to identification of the proteins that may play a role in the virulence factor of Wangiella dermatitdis.