Identification of signature compounds for breast cancer via nuclear magnetic resonance.

Abstract

Chloroform methanol extracts from breast tissue were analyzed via 31p, 1H, and 13C NMR. Tissue samples were obtained from the frozen section room at Saint Elizabeth Medical Center, Youngstown, Ohio. The extraction procedure was a chloroform methanol extraction that resulted in two layers, an chloroform layer and an aqueous layer. Via a series of changes in protocol, the chloroform layer revealed the presence of triglycerides of arachidonic acid and linoleic acid. During the many changes in the aqueous phase of the extraction procedure, a couple of peaks appeared via 31p NMR analysis. One of the peaks was confirmed to be phosphoethanolamine and the other more commonly occurring peak was attributed to be inorganic phosphate.