dc.contributor.author |
Skornicka, Erin L. |
en_US |
dc.date.accessioned |
2011-01-31T14:17:49Z |
|
dc.date.accessioned |
2019-09-08T02:33:02Z |
|
dc.date.available |
2011-01-31T14:17:49Z |
|
dc.date.available |
2019-09-08T02:33:02Z |
|
dc.date.created |
1996 |
en_US |
dc.date.issued |
1996 |
en_US |
dc.identifier |
235467281 |
en_US |
dc.identifier.other |
b17669686 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1766968 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6175 |
|
dc.description |
vii, 55 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 1996. |
en_US |
dc.description |
Includes bibliographical references (p. 48-51) |
en_US |
dc.description.abstract |
Cellular adhesion molecules (CAMs) play an important
role in the inflammatory response by allowing white blood
cells to adhere to the endothelium and then migrate into
infected tissues. The goal of this study is determine the
effect of blood collection techniques on the ability to
detect changes in these molecules. In this study, the
effects of time (0 hour versus 2 hours), temperature (room
temperature versus 4°C), and anticoagulants (EDTA,
potassium oxalate, sodium citrate, and heparin) were
examined to see which blood collection techniques retained
accurate results when compared to samples that were
processed immediately. Cells, both unstimulated and
stimulated with PMA, from healthy volunteers were labeled
with various antibodies against CAMs (anti-ICAM-l, LFAlbeta,
L-selectin, Mac-l, LFA-lalpha, and VLA-4) followed
by FITC-Iabeled goat anti-mouse antibody then analyzed
using a flow cytometer. From these experiments, the time
of incubation and temperature of storage seem to influence
the expression of CAMs. The anticoagulant does not seem to
play an important role in altering CAM expression. In
these studies, the best storage medium for monocytes when
examing Mac-l expression was sodium citrate on ice. |
en_US |
dc.description.sponsorship |
Youngstown State University. Dept. of Biology. |
en_US |
dc.description.statementofresponsibility |
by Erin L. Skornicka. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0564 |
en_US |
dc.subject.classification |
Master's Theses no. 0564 |
en_US |
dc.subject.lcsh |
Monocytes. |
en_US |
dc.subject.lcsh |
Blood--Collection and preservation. |
en_US |
dc.title |
The effect of blood collection methods on the expression of monocyte cellular adhesion molecules by Erin L. Skornicka. |
en_US |
dc.type |
Thesis |
en_US |