dc.contributor.author |
Awad, Keytam. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:17:56Z |
|
dc.date.accessioned |
2019-09-08T02:29:48Z |
|
dc.date.available |
2011-01-31T14:17:56Z |
|
dc.date.available |
2019-09-08T02:29:48Z |
|
dc.date.created |
2001 |
en_US |
dc.date.issued |
2001 |
en_US |
dc.identifier |
49554763 |
en_US |
dc.identifier.other |
b18924384 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1892438 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6182 |
|
dc.description |
iii, 48 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2001. |
en_US |
dc.description |
Includes bibliographical references (leaves 45-48). |
en_US |
dc.description.abstract |
These studies investigated the role of the protein kinase cdk1 (cdc2) with regard
to the cytoskeleton of cultured C2C12 skeletal muscle myoblasts. The cyclin-dependent
protein kinases (cdks) are involved in regulating entry into and progression through
various stages of the cell cycle. The cell cycle consists of four precise phases that
dividing cells pass through: M, G1, S, and G2 phase. Cdc2, a member of the cdk family,
is known for its role in facilitating the transition state from G2 to M phase. In order to
examine the possibility that cdc2 is involved in cytokinesis two factors must be
addressed. First, synchronization of C2C12 cells is critical to ensure that all cells are in
the same phase of M phase before extraction of the cytoskeleton can occur. Establishing
the presence of cdc2 during mitosis leads to the examination of its location and with what
it is specifically linked to. Secondly, the removal of actin, a cytoskeletal protein, as well
as nucleic acids is essential to determine the connection between cdc2 and actin or cdc2
and chromatin. This is important because actin and myosin are key components of the
contractile ring that aids in the process of cytokinesis. If cdc2 is associated with actin,
then cdc2 can possibly playa role in cytokinesis. After synchronization of C2C12 cells
at mitosis, the cytoskeleton was isolated using detergent extraction protocols. Protein
composition was analyzed by Western Blot to investigate the presence of cdc2 in the
cytoskeleton fractions with and without actin and chromatin. Ultimately, the aim of this
study was to show that cdc2 plays a part in cytokinesis by proving that cdc2 is linked to
the cytoskeletal protein actin. |
en_US |
dc.description.statementofresponsibility |
by Keytam Awad. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0737 |
en_US |
dc.subject.classification |
Master's Theses no. 0737 |
en_US |
dc.subject.lcsh |
Protein kinases. |
en_US |
dc.subject.lcsh |
Myoblasts. |
en_US |
dc.title |
Association of cell cycle protein kinases with the cytoskeleton of cultured myoblasts / |
en_US |
dc.type |
Thesis |
en_US |