dc.contributor.author |
Stevic, Bojana. |
en_US |
dc.contributor.author |
Youngstown State University. Dept. of Biology. |
en_US |
dc.date.accessioned |
2011-01-31T14:18:44Z |
|
dc.date.accessioned |
2019-09-08T02:32:06Z |
|
dc.date.available |
2011-01-31T14:18:44Z |
|
dc.date.available |
2019-09-08T02:32:06Z |
|
dc.date.created |
2002 |
en_US |
dc.date.issued |
2002 |
en_US |
dc.identifier |
51556346 |
en_US |
dc.identifier.other |
b19036590 |
en_US |
dc.identifier.uri |
http://jupiter.ysu.edu/record=b1903659 |
en_US |
dc.identifier.uri |
http://hdl.handle.net/1989/6234 |
|
dc.description |
vii, 83 leaves : ill. ; 29 cm. |
en_US |
dc.description |
Thesis (M.S.)--Youngstown State University, 2002. |
en_US |
dc.description |
Includes bibliographical references (leaves 81-83). |
en_US |
dc.description.abstract |
The mechanisms of mitochondrial DNA (mtDNA) replication are not completely
understood. The major current model of mtDNA replication involves an asynchronous,
asymmetric process called D-loop replication. The D-loop model of replication requires
mtTFA and RNA polymerase for initiation of replication. The nuclear gene, (RP041)
encodes catalytic subunit of the RNA polymerase. Massive deletions in mtDNA (rho)
can produce small mtDNA genomes that contain presumed replication origins, called
ori/rep sequences or not. Those rho" genomes that have ori/rep sequences exhibit a
replication/segregation advantage and are called hypersuppressive (BS rho"). Those
without ori/rep sequences are called neutral, rho". All rho· mutants with deleted RP041
decrease the transcription to non-detectable levels, but mtDNA is stably maintained. This
indicates that D-loop mode is not only replication mechanism found in Saccharomyces
cerevisae rho" cells. Two dimensional gel electrophoresis of HS and neutral rho· mtDNA
reveals evidence that another mode of replication is possibly found in these cells. The
presence of long strands of single stranded mtDNA in rho· and wildtype cells grown to
log phases may indicate rolling-circle mechanism of replication in Saccharomyces
cerevisae cells.
Mammalian cells have been found to have two modes of mtDNA replication. An
asymmetric-asynchronous D-loop mechanism of mtDNA replication is found in cells that
maintain their copy number of mtDNA. Another mode of mtDNA replication was
identified in cells that need to reamplify the copy number of mtDNA. Analogous to these
findings this study results suggests different modes of replication in cells that are rapidly
increasing their mtDNA copy number (cells in log phase), possibly through a rolling circle
mechanism of replication. Another mode of replication is found in stationary cells
that simply maintain their mtDNA copy number, possibly by a D-loop mode of
replication. |
en_US |
dc.description.statementofresponsibility |
by Bojanna Stevic. |
en_US |
dc.language.iso |
en_US |
en_US |
dc.relation.ispartofseries |
Master's Theses no. 0766 |
en_US |
dc.subject.classification |
Master's Theses no. 0766 |
en_US |
dc.subject.lcsh |
Mitochondrial DNA. |
en_US |
dc.subject.lcsh |
Saccharomyces cerevisiae. |
en_US |
dc.title |
Replication analysis of mitochondrial DNA in the yeast saccharomyces cerevisae / |
en_US |
dc.type |
Thesis |
en_US |