Proteomic profiling of Bacillus subtilis with tylsoin [i.e. tylosin] /

Abstract

Bacillus subtilis is a Gram-positive bacterium capable of producing a wide range of industrially important enzymes and antibiotics; making it a perfect subject for proteomicdrug analysis. Here, B. subtilis will be grown with a minimum inhibitory concentration of the macrolide class antibiotic Tylosin, which inhibits protein synthesis. The results were compared to untreated B. subtilis control samples. Through two-dimensional gel analysis and digital imaging software, it is seen that acidic shifting occurred in the treated proteome expression profile indicating a chemical modification, via new isoelectric points, during the exposure of B. subtilis to Tylosin.