Investigation of 9-Anthryldiazomethane as a fluorescent label for nucleotides, by Antoinette L. Vergallito .

Vergallito, Antoinette L.; Youngstown State University. Dept. of Chemistry.

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dc.contributor.author	Vergallito, Antoinette L.	en_US
dc.contributor.author	Youngstown State University. Dept. of Chemistry.	en_US
dc.date.accessioned	2011-01-31T14:20:35Z
dc.date.accessioned	2019-09-08T02:33:04Z
dc.date.available	2011-01-31T14:20:35Z
dc.date.available	2019-09-08T02:33:04Z
dc.date.created	1997	en_US
dc.date.issued	1997	en_US
dc.identifier.other	b1788164x	en_US
dc.identifier.uri	http://jupiter.ysu.edu/record=b1788164	en_US
dc.identifier.uri	http://hdl.handle.net/1989/6341
dc.description	x, 60 leaves ill. ; 29 cm.	en_US
dc.description	Thesis (M.S.)--Youngstown State University, 1997.	en_US
dc.description	Includes bibliographical references (leaves ).	en_US
dc.description.abstract	Currently, biotin is the most used nonradioactive label for nuc1eotides. This paper examines the use of9-anthryldiazomethane (ADAM) as an alternate method for labeling nucleotides, extending its use as a fluorescent labeling reagent to include phosphorylated as well as carboxyl containing biomolecules. Adenosine 5'-monphosphate (AMP) was the primary nucleotide investigated. Studies were also conducted on the synthesis and purification ofADAM. A wide variety oftechniques were used in this project. Thin layer chromatography was used to monitor reactions. Attempts to separate the mixture after synthesis ofADAM utilized gravity, flash, and high pressure liquid chromatography. Mass spectrometry and 31p NMR were used to test for the presence of an ADAM-AMP derivative.	en_US
dc.language.iso	en_US	en_US
dc.relation.ispartofseries	Master's Theses no. 0588	en_US
dc.subject.classification	Master's Theses no. 0588	en_US
dc.subject.lcsh	Theses (Master's)	en_US
dc.title	Investigation of 9-Anthryldiazomethane as a fluorescent label for nucleotides, by Antoinette L. Vergallito .	en_US
dc.type	Thesis	en_US

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